General Cell Based Assays
Eurofins Discoverx AssayComplete Cell Plating 27 Reagent, 500. (93-0563R27B)
AssayComplete Cell Plating 27 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress EDG7 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0636E2CP13L)
The PathHunter EDG7 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. LPA) based activation of EDG7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged EDG7 and EA-tagged β-Arrestin-2. Activation of EDG7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
American Research Products Inc Mouse Macrophage Migration Inh
Mouse Macrophage Migration Inhibitory Factor MIF ELISA Kit from CUSABIO Detection Range 125 pg/ml - 8000 pg/ml Sensitivity 31 25 pg/ml Sample serum plasma tissue homogenates Method Sandwich
Eurofins Discoverx PathHunter eXpress PROKR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0282E2CP2M)
The PathHunter PROKR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prokinectin, EG-VEGF) based activation of PROKR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PROKR2 and EA-tagged β-Arrestin-2. Activation of PROKR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 32 Reagent, 500. (93-0563R32B)
AssayComplete Cell Plating 32 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress BAI3 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0547E2ACP1L)
The PathHunter BAI3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of BAI3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged BAI3 and EA-tagged β-Arrestin-2. Activation of BAI3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GALR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0228E2CP0L)
The PathHunter GALR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. galanin) based activation of GALR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GALR1 and EA-tagged β-Arrestin-2. Activation of GALR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
ABclonal Technology HRP-conjugated B-Actin
This gene encodes one of six different actin proteins Actins are highly conserved proteins that are involved in cell motility structure integrity and intercellular signaling The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed Mutations in this gene cause Baraitser-Winter syndrome 1 which is characterized by intellectual disability with a distinctive facial appearance in human patients Numerous pseudogenes of this gene have been identified throughout the human genome
ABclonal Technology HRP-conjugated B-Actin
This gene encodes one of six different actin proteins Actins are highly conserved proteins that are involved in cell motility structure integrity and intercellular signaling The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed Mutations in this gene cause Baraitser-Winter syndrome 1 which is characterized by intellectual disability with a distinctive facial appearance in human patients Numerous pseudogenes of this gene have been identified throughout the human genome
Cayman Chemical FFA asy Cofactr 3 1ea
Wash Buffer Concentrate (400X) has been formulated and tested to work with Cayman’s Assay Kits. Detailed instructions for its use are contained in the respective Cayman Assay Kit booklet.
Enzo Life Sciences GFP-CERTIFIED FLUOFORTE Calcium assay kit (10x96wells)
Enzo Life Sciences’ GFP-CERTIFIED FLUOFORTE Calcium Assay Kit (high-throughput) provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets. Relative to other commercially available dyes, the GFP-CERTIFIED FLUOFORTE dye is the brightest and most sensitive fluorescent calcium indicator. The kit provides a homogeneous mix-and-read, no-wash calcium mobilization assay. The homogenous cell-based assay for calcium offers fewer steps, lower variability and an easier protocol for adherent and non-adherent cell lines. In addition, it requires neither a washing step, nor exogenous addition of a quencher dye, which could adversely effect receptor-ligand interaction kinetics. Applications: Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS .
Eurofins Discoverx cAMP Hunter eXpress PTHR2 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0057E2CP2M)
The cAMP Hunter PTHR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. parathyroid hormone, TIP 39) based activation of PTHR2 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTHR2, a naturally Gs coupled GPCR. Upon activation of PTHR2, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress CHRM2 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0078E2CP2M)
The cAMP Hunter CHRM2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. oxotremorine) based activation of CHRM2 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CHRM2, a naturally Gi/Go coupled GPCR. Upon activation of CHRM2, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cell Biolabs Inc Total Bile Acid Assay Kit (Fluorometric), 100 assays
Determining circulatory levels of bile acids can be used to identify or diagnose certain liver diseases. In addition, elevated serum bile levels have been observed in intrahepatic cholestasis of pregnancy cases. Cell Biolabs’ Total Bile Acid Assay Kit is a simple fluorometric assay that measures the amount of total bile acid present in plasma, serum, tissue homogenates, or cell lysates in a 96-well microtiter plate format. The assay is based on an enzyme driven reaction: when bile acids are incubated in the presence of 3α-hydroxysteroid dehydrogenase (3α-HSD) and NAD+, NAD+ is converted to its reduced form NADH. Diaphorase then uses NADH to reduce resazurin to resorufin which is then detected fluorometrically. Sample bile acid concentrations are determined by comparison with a known bile acid standard. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, bile acid standards and unknown samples.